FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001648559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649594
Sequences flagged as poor quality0
Sequence length35-301
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA32575550.14747673162005No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG18741228.850635935676745No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCGGATTTGGGTGTTTT106941.646259047959187No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA59840.9211907745453314No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG34460.5304851953681838No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAAAGTTA31720.48830500281714423No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAGAGTTA27360.421186156276074No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCATCGTTGAAAATTT22400.3448307712201775No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA18330.2821762516279399No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTG17030.26216375151248317No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT16630.2560060591692657No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTT16010.24646163603727866No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG13900.2139798089268066No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTTGATAGTTA13570.20889971274365218No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGGTG12890.19843163576018252No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTG12290.18919509724535633No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG9130.14054932773393844No Hit
GCTGCGTTCTTCACCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA8770.13500740462504274No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA7640.1176119237554534No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAGAGGTA7490.11530278912674685No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGGTA7470.114994904509586No Hit
GCTGCGTTCTTCATCGATGTCAGAGCCAAGAGATCCGTTGTTGATAGTTA7410.11407125065810338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)