FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592711
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG15205825.654661377973415No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA11468219.348721383608538No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC7755113.084116879895936No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA443627.484591984964005No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG346925.853105476362004No Hit
TCCGTAGGTGAACCTGCGTTGGGATCATTTTGTTTGATATTTTGGCTATG148602.507124045276703No Hit
TCCGTAGGTGAACTCTTGGGCGGGGGGGGGGACGTGCCGCGCCAGGAAGG32930.5555827376242385No Hit
TCCGTAGGTGAACCTGCCGCCCGCCGCCAGAGGCCGAGGCCAGACGATCT26810.45232836913774166No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC19790.33388953469734833No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTTACTATACTTTTG19490.3288280460460494No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC16740.28243106674247653No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTATTGATTTGACTATACTTTTG16130.27213937315150216No Hit
TCCGTAGGTGAACTCTTGGGCGGGGGGGGGGGACGTGCCGCGCCAGGAAG12070.2036405600705909No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG12070.2036405600705909No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11070.186768931232928No Hit
TCCGTAGGTGAACCTGCTGAGGCCAGGAGAAGGAGGTCATAGGGCAGCAG10230.1725967630092912No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG9570.1614614879764337No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGCTTTGGGCAGGCCA8730.14728931975279688No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTTGGCAGGCCA7680.12957410947325088No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGCTTCAAGGGCCAGC6880.11607680640312058No Hit
TCCGTAGGTGAACTCTTGGGCGGGGGGGGGACGTGCCGCGCCAGGAAGGG6280.10595382910052284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)