FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592711
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA15834126.71470581784377No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG12914621.789033778688097No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG12540921.158541009024635No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG266204.4912275965858575No Hit
GCTGCGTTCTTCATCGAAACGAAAGCCTAGTTCTCCACCATAACGAGTAA155402.621851121372811No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA29590.49923149730644445No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT27830.4695374305521578No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG20180.3404694699440368No Hit
GCTGCGTTCATTCGTCGTCTGCGCTTCAGATGCACGGCACTGAGACCCTT17860.301327291040659No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA17830.3008211421755291No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAAAGTTA15700.26488457275130717No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG15630.26370355873267076No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAGAGTTA14080.23755253403429324No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG13990.23603408743890364No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA11530.19452988049825296No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10590.17867054939084984No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG10190.17192189785578468No Hit
GCTGCGTTCTTCATCGATGCCACTGTGCTTTCTCCCAGCCAGGCCCTACT9830.16584811147422604No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT9730.16416094859045977No Hit
GCTGCGTTCTTCATCGATGCCCGAGCCGAGTGATCCACCGCTAAGAGTCG8620.14543344058065397No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT8190.13817864018045894No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGTCG7650.12906796060812098No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTT7490.1263684999940949No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT7460.12586235112896504No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTTGATAGTTA7300.12316289051493899No Hit
GCTGCGTTCTTCATCGATGGCAGAACCAAGAGATCCGTTGTCGAAAGTTG7010.11827011815201675No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGATAGTTG6850.1155706575379907No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGCCGAAAGTTG6800.11472707609610755No Hit
GCTGCGTTCTTCATCGTCTGCGCTTCAGATGCACGGCACTGAGACCCTTG6400.1079784245610424No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGCGTCG6270.10578511281214623No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTACGAGTCG6130.10342308477487341No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)