FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661026
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG27939642.26702126693957No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA9430314.26615594545449No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGTGACCCTGACCAAAACA8665213.108712819162937No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG182002.7532956343623396No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67921.0274936235488468No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTTACTATACTTTTG29230.4421913812769846No Hit
TCCGTAGGTGAACCTGCGGATGTAGCATCGATGAAGAACGCAGC28940.43780426185959403No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTATTGATTTGACTATACTTTTG28920.437501701899774No Hit
TCCGTAGGTGAACCTGCGGATGTAATGAAGAACGCAGC28680.43387098238193356No Hit
TCCGTAGGTGAACCTGCGGATGTAGATGAAGAACGCAGC27740.41965066427039177No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGGGACCCTGACCAAAACA26930.4073969858976803No Hit
TCCGTAGGTGAACCTGCGGATGTAGCGATGAAGAACGCAGC26290.3977150671834391No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG21980.332513395842221No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGTGACCCTGACCACAACA20860.31557003809229894No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG19330.2924242011660661No Hit
TCCGTAGGTGAACCTGCGGATGTAGTCGATGAAGAACGCAGC19070.2884909216884056No Hit
TCCGTAGGTGAACCTGCGGATGTGATGAAGAACGCAGC18890.28576788205002523No Hit
TCCGTAGGTGAACCTGCGGAGCATCGATGAAGAACGCAGC18220.27563212339605403No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA16430.24855300699216068No Hit
TCCGTAGGTGAACCTGCGGCGATGAAGAACGCAGC14190.21466629149231647No Hit
TCCGTAGGTGAACCTGCGGATGTAGAATGAAGAACGCAGC13390.202563893099515No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGTCTATACTTTTG12810.1937896542647339No Hit
TCCGTAGGTGAACCTGCGGATGTAGAAGATGAAGAACGCAGC11230.16988741743895097No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTGTCGTGACCCTGACCAAAACA9890.14961590013100845No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAGACCCTGACCAAAACA8640.13070590264225612No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGCTTTGGGCAGGCCA8350.1263187832248656No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTCTACTTTTG7670.11603174459098432No Hit
TCCGTAGGTGAACCTGCGGAAGGAGCATTATTGATTTGACTATACTTTTG7660.1158804646110743No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTTGGCAGGCCA7220.10922414549503348No Hit
TCCGTAGGTGAACCTGCGGATGTAGAGATGAAGAACGCAGC6700.10135758653971251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)