FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661026
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA29507144.63833495202912No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG9934815.029363444100536No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG9447314.291873542039193No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG92141.393893734890912No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN64860.9812019496963811No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA52210.7898327751102074No Hit
GCTGCGTTCTTCATCGATGCTACATCCGCAGGTTCACCTACGGA30650.4636731384242072No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAAAGTTA30260.4577732192077165No Hit
GCTGCGTTCTTCATCTACATCCGCAGGTTCACCTACGGA29280.4429477811765347No Hit
GCTGCGTTCTTCATTACATCCGCAGGTTCACCTACGGA29080.4399221815783343No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG27890.4219198639690421No Hit
GCTGCGTTCTTCATCGCTACATCCGCAGGTTCACCTACGGA26360.39877402704280923No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAGAGTTA25740.3893946682883881No Hit
GCTGCGTTCTTCATCACATCCGCAGGTTCACCTACGGA20380.30830859905661806No Hit
GCTGCGTTCTTCATCGACTACATCCGCAGGTTCACCTACGGA20350.307854759116888No Hit
GCTGCGTTCTTCATCGATGCTCCGCAGGTTCACCTACGGA18340.27744748315497425No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA16100.24356076765513004No Hit
GCTGCGTTCTTCATCGCCGCAGGTTCACCTACGGA13670.20679973253699552No Hit
GCTGCGTTCTTCATTCTACATCCGCAGGTTCACCTACGGA13360.20211005315978495No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTT12490.18894869490761332No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTTGATAGTTA12230.18501541542995284No Hit
GCTGCGTTCTTCATCTTCTACATCCGCAGGTTCACCTACGGA11990.1813846959121124No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG9490.14356470093460771No Hit
GCTGCGTTCTTCACCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA9190.13902630153730716No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGAGATCCGTTGCCGAGAGTCG8900.1346391821199166No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAGAGGTA8220.12435214348603535No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCT7790.11784710434990454No Hit
GCTGCGTTCTTCATCTATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG7560.11436766481197412No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGGTA7530.11391382487224405No Hit
GCTGCGTTCTTCATCTCTACATCCGCAGGTTCACCTACGGA7130.10786262567584332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)