FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001648564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57220
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAGAAAGGGGCTTAAAAA1741130.428171967843408No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA1124419.65047186298497No Hit
TCCGTAGGTGAACCTGCGGAGGGGTCATTACAAGTGACCCCGGTTTACCA53549.356868227892345No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCTTCAGTGGTTTTCCACTG47318.268088081090527No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAGTTAGGGTCCTAGTGG44687.808458580915764No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTGTAAAAACTCCCAA16822.9395316322964No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC13582.3732960503320517No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG10721.8734708144005592No Hit
TCCGTAGGTGAACCTGCATGAGGCAGCGGTCCCTCAGAGAGTCTGTTTCT3640.6361412093673541No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC3280.5732261447046487No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCCGGCCA2810.49108703250611674No Hit
TCCGTAGGTGAACCTGCGGCTTCCTGGGAGAGAGGAGAAAAGTCAAAGGC2730.47710590702551553No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA2580.4508912967493883No Hit
TCCGTAGGTGAACCTCGGTGGGTAGGATTCAGAGGGGCTTTTGGCTTCCC2400.4194337644180357No Hit
TCCGTAGGTGAACCTGTAGGGATTGGGCAGCTCCGTGAGTGTCTCTGTCG1940.3390422929045788No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1560.27263194687172315No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGAGAAAGGGGCTTAAAAA1250.2184550856343936No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAGAAATGGGCTTAAAAA1230.21495980426424324No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCGGCCGGTGCATCGATGAAGAACGCAGC950.16602586508213912No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTTGGCAGGCCA930.16253058371198884No Hit
TCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTATCCCTACCTGATCC810.14155889549108705No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAGAAAGGGGCTTCAAAA790.13806361412093673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)