FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001648568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50897
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA931618.303632827082147No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC929518.262373027879836No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCGAGGCAGAC681813.395681474350157No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAATGCCTGGGCTGTCAT37567.379609800184686No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCTGGG35426.959152798789712No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCTAGCAGAA27755.452187751733894No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCTTCAGTGGTTTTCCACTG26315.169263414346622No Hit
TCCGTAGGTGAACCTGCGGAAGTATCATTATCTTCAGTGGTTTTCCACTG26185.143721633888049No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAAACATCAAA24314.776312945753188No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG5671.1140145784623847No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2040.4008094779653025No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCCGGCCA2020.3968799732793681No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA1940.3811619545356308No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC1930.3791972021926636No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTGTAAAAACTCCCAA1610.3163251272177142No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATGCCTGGGCTGTCAT1100.2161227577263886No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAAACCTCAAA770.15128593040847202No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTTGGCAGGCCA720.14146216869363618No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCTCGCAGAA630.12377939760693166No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)