FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001648569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50897
Sequences flagged as poor quality0
Sequence length35-301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG2323945.65887969821404No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1190823.396270900053047No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG524310.301196534176867No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT36677.204746841660609No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG4280.8409140027899484No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2080.4086684873371712No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG1910.3752676975067293No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCATTGCTGAAAGTTT1690.33204314596145157No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG940.1846867202389139No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGCAAGTTG610.11984989292099732No Hit
GCTGCGCTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG530.10413187417725996No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG510.10020236949132562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)