FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001648571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102759
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT5075949.396159947060596No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG100589.787950447162778No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA88668.627954729026168No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA53695.224846485465993No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG48154.6857209587481385No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCAAGAGATCCATTGTTAAAAGTTG43464.229313247501435No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG38593.7553888223902527No Hit
GCTGCGTTCTTCATCGATGCGCTGACCGATCTAGTGCCCGCAGACTCACG36123.5150205821387908No Hit
GCTGCGTTCTTCATCGATGCGAGAACCTAGATATCCATTGCTAAGAGTTG8900.8661041855214627No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG6430.6257359452700006No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT2070.20144220944150878No Hit
GCTGCGTTCTTCAACGAAATAGGAAGCCAAGTCATCCATCGCGACACGTT2050.1994959079010111No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGCTGAAAGTTT1890.18392549557702975No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG1530.1488920678480717No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT1510.14694576630757403No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT1400.13624110783483687No Hit
GCTGCGTTCTTCATCGATTCGAGAACCAAGAGATCCGTTGTTGAAAGTTT1300.1265096001323485No Hit
GCTGCGTTCTTCATCGAAATAGGAAGCCAAGTCATCCATCGCGACACGTT1150.11191233857861599No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG1110.10801973549762064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)