FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001648572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48579
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACC1936439.86084522118611No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG859217.686654727351325No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCAAAGCAGAA29626.097284835011013No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCAAGGCAGAA26455.444739496490253No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTTTACCA24675.078326025648943No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGAGCAAGGGTCCTCACCG23924.923938327260751No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG6741.387430782848556No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGATTGT6691.3771382696226764No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC4190.8625126083287017No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA3970.8172255501348319No Hit
TCCGTAGGTGAACCTGCAGCTGGATCATTTCGAAAGTTTTTAAAAAACTT3800.7822310051668416No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCACATCTCTAAACAAATCA3110.6401943226497047No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGCCAAATTATAATTCTGT2900.5969657671010108No Hit
TCCGTAGGTGAACCTGCGGAATCTGATGATAAAGCTTTAACATCGTAAAT2790.5743222380040759No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC2380.48992362955186397No Hit
TCCGTAGGTGAACCTGCCCATTGGCATCGATGAAGAACGCAGC2120.4364025607772906No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1510.31083389942156076No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC1250.2573128306469874No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG1250.2573128306469874No Hit
TCCGTAGGTGAACCTGCCCAGCATCGATGAAGAACGCAGC1220.2511373227114597No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTTTAACTCTCCAA970.1996747565820622No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCAACGCAGAA880.18114823277547912No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTTCACC850.17497272483995144No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCCCC520.10704213754914675No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)