FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001648573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48579
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT1979740.75217686654727No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA870217.913090018320673No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG558411.49467877066222No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT489910.08460445871673No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAAAAAAATACTACCAAACTG6571.3524362378805657No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG4730.9736717511682003No Hit
GCTGCGTTCTTCATCGACCCGAAAGCCGAGACATCCACCGCCATGAGTTG4250.874863624199757No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC4080.8398690792317668No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG3750.7719384919409622No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCACCGTTGAAAATTT3100.6381358200045287No Hit
GCTGCGTTCTTATCAGACGATGGCAAATTAATGATTGATGATCAACAATA2450.5043331480680953No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA2330.4796311163259845No Hit
GCTGCGTTCTTCATCGATGCCAATGGGCAGGTTCACCTACGGA2140.4405195660676424No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCGTTGCTGAAAGTGT1700.3499454496799029No Hit
GCTGCGTTCTTCATCGTTGTGGGAGCCAAGACATCCGTTGCTGAAAGTGT1630.3355359311636716No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA1600.32936042322814385No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1520.3128924020667367No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA1210.2490788200662838No Hit
GCTGCGTTCTTCATCGATGCTGGGCAGGTTCACCTACGGA1190.24496181477593199No Hit
GCTGCGTTCTTCATCGATGCCCGAACCAAGAGATCCGTTGTTGAAAGTTT1040.2140842750982935No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGCTGAAAGTTT720.14821219045266473No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG710.14615368780748883No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT710.14615368780748883No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAAAACAATACTACCAAACTG530.10910064019432265No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA500.10292513225879496No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)