FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001648574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892540
Sequences flagged as poor quality0
Sequence length35-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGTTTGCTTAATTGCATTA29455433.00177022878527No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC25335328.385618571716677No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG867099.714858717816568No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG125331.4041947699822976No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC72400.8111681269186816No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGGTTGCTTAATTGCATTA70150.7859591726981424No Hit
TCCGTAGGTGAACCTGCGGAATCTGATGATAAAGCTTTAACATCGTAAAT64980.7280345978891702No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC36630.4104017747103771No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGATTGCTTAATTGCATTA30720.344186254957761No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAAAGTTTGCTTAATTGCATTA30580.3426176978062608No Hit
TCCGTAGGTGAACGCACAAGCAGCAGAACGCCAAGTGAACGCACGAGGGG26000.29130347099289666No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGTGCCAGC24150.2705761086337867No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGTTTGCTTAATTGCATTA22100.24760795034396216No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGCTTCAAGGGCCAGC21960.24603939319246196No Hit
TCCGTAGGTGAACCTGCCGAAGGATCATTAGTGAAGATTCAAGGGCCAGC16980.19024357451766866No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGTTTTCTTAATTGCATTA15540.1741098438165236No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGTTTGCTTAATTGCCTTA15030.16839581419320143No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGTTTGCTTAATTGCATTA14150.15853631209805724No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGTCCAGC13160.14744437224102No Hit
TCCGTAGGTGAACCTGCATGAGGCAGCGGTCCCTCAGAGAGTCTGTTTCT12080.13534407421516123No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGTTTGCTTAATTTCATTA10610.11887422412440898No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTTACTATACTTTTG9970.11170367714612231No Hit
TCCGTAGGTGAACCTGCAGCAGGATCATTAGTGAAGATTCAAGGGCCAGC9810.10991104040155063No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGCGCCAGC9330.10453313016783562No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGTTTGCTTAATTGCATCA9300.10419701077822843No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCCAGGGCCAGC9270.10386089138862124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)