FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001648575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892540
Sequences flagged as poor quality0
Sequence length35-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT34034638.13229659174939No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG27295230.581486544020436No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA8938110.014229054160037No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG83130.9313868286015192No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC74390.8334640464292917No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG70170.7861832522912138No Hit
GCTGCGTTCTTATCAGACGATGGCAAATTAATGATTGATGATCAACAATA53910.6040065431241177No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA38620.4326976942209873No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG32300.3618885428104063No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT31840.3567347121697627No Hit
GCTGCGTTCTTCCCCCTGTGTGTTCGCGCGGCGTTATTTCCCCTGTGTGC24220.2713603872095368No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT22750.25489053711878457No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT19640.22004616039617272No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG17450.19550944495484798No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGGTT17320.19405292759988346No Hit
GCTGCGTTCTTCATCGATTGGAGAACCAAGAGATCCGTTGTCGAAAGTTG15490.173549644833845No Hit
GCTGCGTTCTTCATCGATGGCAGAACCAAGAGATCCGTTGTCGAAAGTTG14210.1592085508772716No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGATAGTTT13950.15629551616734264No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG13420.15035740695094896No Hit
GCTGCGTTCTTCATCCCTCTGTGTAGCACATGGACTGCCCACACTCAGGG11810.13231899970869654No Hit
GCTGCGTTCTTCATCGATGGCAAATTAATGATTGATGATCAACAATAGTA11110.12447621395119547No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTA9940.1113675577565151No Hit
GCTGCGTTCTTCACCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT9540.10688596589508592No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAAAGTTA9330.10453313016783562No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT9270.10386089138862124No Hit
GCTGCGTTCTTCATCGATGCACCGGCCGCTCCACAATGGGCAGGTTCACCTACGGA9230.10341273220247833No Hit
GCTGCGTTCTTCATCGGTATGAAACCTTCACAGATCACTTTCCTCAGTCT8960.10038765769601363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)