FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001648576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833494
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA15941619.126232462381253No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG12721015.262257436766191No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC11927514.31024098553799No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCTTCAGTGGTTTTCCACTG750659.006063630931957No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTGTAAAAACTCCCAA706938.481524762025881No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGCCTTCGGGCTAA405774.868301391491721No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG310863.7296009329401287No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTATCTTA244552.9340343181834543No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACGCCAATTATTCTTCTTGC43200.5183000717461673No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA34240.4108007976062215No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC27190.32621710534209003No Hit
TCCGTAGGTGAACCTGCCCATGCATCGATGAAGAACGCAGC18680.22411679028283346No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC17480.20971956606766215No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTATTGATTTGACTATACTTTTG12080.14493205709939122No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGCTTTGGGCAGGCCA11920.14301242720403506No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTTACTATACTTTTG11840.14205261225635699No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTTGGCAGGCCA11470.13761346812334582No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCTGCAGTGGTTTTCCACTG9910.1188970766436231No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG9800.11757733109056573No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGTGCCAGC9530.11433795564215218No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG9420.11301821008909481No Hit
TCCGTAGGTGAACCTGCCGAAGGATCATTAGTGAAGATTTGGGCAGGCCA9040.10845908908762389No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGCTTCAAGGGCCAGC8910.10689938979764702No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCCGGCCA8340.10006070829544063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)