FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001648577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833494
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG29444135.326109126160475No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA13285815.939886789826922No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG813069.754839266989325No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT749528.992506244796003No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA440095.28006200404562No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG258873.105841193817832No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG233742.8043393233784526No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG80210.9623344619157426No Hit
GCTGCGTTCTTCATCGATATGGGAACCTAGATATCCATCGTTGAAAATTT44310.5316175041452008No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA25380.3045012921508733No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG23810.2856649238026908No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT19160.22987567996890199No Hit
GCTGCGTTCTTCATCGATGCATGGGCAGGTTCACCTACGGA19110.2292757956266032No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT18940.22723618886278726No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG18490.22183722978209802No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT17710.21247903404223667No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA17140.20564035254003027No Hit
GCTGCGTTCTTCATCGATGGCAGAACCAAGAGATCCGTTGTCGAAAGTTG15390.18464440055957212No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG13510.16208874928913705No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAAAGTTA13470.161608841815298No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG13260.15908932757764302No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAGAGTTA10870.13041485601576017No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGACAGTTG9820.11781728482748526No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT9330.11193841827295697No Hit
GCTGCGTTCTTCACCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG9120.109418904035302No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTTGAAAGTTG9070.10881901969300319No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA8420.10102052324311872No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)