FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724968
Sequences flagged as poor quality0
Sequence length35-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATAAGTAGTTTGCTACT48647767.103237660145No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAATCGCGGCGGAGGGGC289853.99810750267598No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG216012.979579788349279No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTATGAATAAGTAGTTTGCTACT53230.7342393043555026No Hit
TCCGTAGGTGAACCTGCGGAAGGATCTGGAGCGGTGGCGCGGGCGACAGT51680.7128590503304973No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG31670.43684686772381676No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATAAGTAGTTTGCTCCT30870.42581189790445917No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATACGTAGTTTGCTACT27020.3727061056488011No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATAAGTCGTTTGCTACT25780.35560190242879686No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA25220.34787742355524653No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATAAGTAGTTTGCTACG23240.32056587325233665No Hit
TCCGTAGGTGAACCTGCGGCAGGATCATTATGAATAAGTAGTTTGCTACT22740.3136690171152382No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTAATAAGTAGTTTGCTACT22680.3128413943787864No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATAATTAGTTTGCTACT22230.30663422385539774No Hit
TCCGTAGGTGAACCTGCGGAGCACGACGGCATCGATGAAGAACGCAGC19940.2750466227474868No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATAAGTAGATTGCTACT18100.24966619216296443No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATAAGTAGTTTGCTACC13450.18552543008794872No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATAAGTAGTTCGCTACT12850.17724920272343056No Hit
TCCGTAGGTGAACCTGCTGAAGGATCATTATGAATAAGTAGTTTGCTACT12460.17186965493649375No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGGATAAGTAGTTTGCTACT12200.16828328974520254No Hit
TCCGTAGGTGAACCTGCGGATGGATCATTATGAATAAGTAGTTTGCTACT12090.16676598139504087No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATTAGTAGTTTGCTACT11140.1536619547345538No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATAAGTAGGTTGCTACT10620.1464892243519714No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATAAGTAGTTTGCTACA10050.13862680835567914No Hit
TCCGTAGGTGAACCTGCGGAGGGCATCGATGAAGAACGCAGC9600.13241963783229052No Hit
TCCGTAGGTGAACCTGCGGAAGGAGCATTATGAATAAGTAGTTTGCTACT9130.12593659306341798No Hit
TCCGTAGGTGAACCTGCGGGGCATCGATGAAGAACGCAGC9090.1253848445724501No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATAAGTAGCTTGCTACT9060.12497103320422417No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATAAGTAGTCTGCTACT8960.12359166197680449No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATAAGTAGTTTTCTACT8950.12345372485406253No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCGGCCGGTGCATCGATGAAGAACGCAGC8410.11600512022599618No Hit
TCCGTAGGTGAACCTGCGGAAGGCTCATTATGAATAAGTAGTTTGCTACT7530.10386665342470289No Hit
TCCGTAGGTGAACCTGCCCATTGGCACGATGAAGAACGCAGC7470.10303903068825107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)