FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001648579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724968
Sequences flagged as poor quality0
Sequence length35-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG53365073.61014555125192No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTGAAAGTTG310194.278671610333146No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA217472.9997186082696063No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT81611.1257048586972116No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG65310.9008673486278015No Hit
GCTGCGTTCTTCATCGATGCGACGTGCACGACGTTGAGCATCGCCGGGGC53080.732170247514373No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG32030.4418126041425277No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTG30720.4237428410633297No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG27220.37546484810364045No Hit
GCTGCGTTCTTCATCGATGCCGTCGTGCTCCGCAGGTTCACCTACGGA19830.2735293143973251No Hit
GCTGCGTTCTTCACCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG17440.24056234206199448No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGGTGAAAGTTG16950.233803423047638No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGATAGTTG16140.22263051610553844No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA12610.17393871177762327No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG12410.1711799693227839No Hit
GCTGCGTTCTTCATCGATGTCAGAACCAAGAGATCCGTTGTTGAAAGTTG12230.16869710111342845No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGATATCCGTTGTTGAAAGTTG10880.15007558954326258No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAACGTTG10600.14621335010648745No Hit
GCTGCGTTCTTCATCGATGCCCTCCGCAGGTTCACCTACGGA10050.13862680835567914No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTCGTTGAAAGTTG10030.1383509341101952No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGCAAGTTG10010.13807505986471125No Hit
GCTGCGTTCTTCATCGATGCCCCGCAGGTTCACCTACGGA9300.12828152415003144No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGACAGTTG8920.12303991348583661No Hit
GCTGCGTTCTTCATCGATGCACCGGCCGCTCCACAATGGGCAGGTTCACCTACGGA8880.12248816499486875No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTG8370.1154533717350283No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTA8130.11214288078922105No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG7590.1046942761611547No Hit
GCTGCGTTCTTCATCGTGCCAATGGGCAGGTTCACCTACGGA7570.10441840191567076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)