FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780716
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA20646226.445211831190857No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGCTAATCCAACAACCTAA11936115.288658103586963No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGCCGTAACCTGCTTAGCAGAA737699.448890505638415No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG653738.373467432459435No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAAATTATCGTGTTCTTTGG606807.7723525584207325No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATAAGTAGTTTGCTACT509426.525035992601663No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG110061.4097315797293766No Hit
TCCGTAGGTGAACCTGCGGAGGGCATCGATGAAGAACGCAGC31490.4033476962173184No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC27310.3498071001490939No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGCCGGAACCTGCTTAGCAGAA24800.3176571249980787No Hit
TCCGTAGGTGAACCTGCGGACTGCATCGATGAAGAACGCAGC22620.28973403901034434No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACCGAGTTTACAACTCCCAAA21520.27564440846607474No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGCCGAAACCTGCTTAGCAGAA17920.22953289032119234No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTTACAACTCCCAAA17800.22799583971636292No Hit
TCCGTAGGTGAACCTGCGGAGCCCCGCATCGATGAAGAACGCAGC17710.22684305176274086No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTAACGCTCTCCATTTAAGTAGT17060.21851736098658153No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAACGCTAATCCAACAACCTAA13150.16843512877922318No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTCCAACTCCCAAA12520.16036561310386874No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGCTAATCCAACCACCTAA10090.12924033835607315No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCCAA9510.121811260432731No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCACA9500.12168317288232854No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGCTAATCCAACACCCTAA7890.1010610772675339No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)