FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001648581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780716
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT21047226.958842908304685No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCATCGTTGAAAATTT11504214.735447973398777No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCCGAGAGTCG8735311.18883179030531No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA680918.721609394453296No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCAAGAGATCCATTGTTAAAAGTTG648528.306733818699758No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG579927.428053222938943No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG56910.7289462493403491No Hit
GCTGCGTTCTTCATCGATGCCCTCCGCAGGTTCACCTACGGA33970.4351134087171264No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA28980.3711977210663033No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT27430.35134415075392333No Hit
GCTGCGTTCTTCATCGATATGGGAACCAAGATATCCATCGTTGAAAATTT27150.34775769934265466No Hit
GCTGCGTTCTTCATCGATGCAGTCCGCAGGTTCACCTACGGA25580.3276479539294699No Hit
GCTGCGTTCTTCATCGGTATGGGAACCAAGATATCCATCGTTGAAAATTT21520.27564440846607474No Hit
GCTGCGTTCTTCATCGATGCAGGAGCCGAGATATCCATCGCTGAGGTTTT19170.24554383412149872No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA18060.23132611602682665No Hit
GCTGCGTTCTTCATCGATGCGGGGCTCCGCAGGTTCACCTACGGA17610.22556217625871638No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAACGTTT14990.19200323805327418No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGATAGTTT14770.18918531194442026No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT13910.17816978260980945No Hit
GCTGCGTTCTTCATCGTTCTGGGAACCAAGATATCCATCGTTGAAAATTT13750.17612038180337025No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGGTT13530.1733024556945163No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT12400.15882856249903934No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG11250.14409849420275747No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGACAGTTT10860.13910307973706187No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGCAAGTTT9610.12309213593675548No Hit
GCTGCGTTCTTCATCGATTCCAGAACCAAGAGATCCGTTGTTGAAAGTTT9330.11950568452548686No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCATCGTTGAAAGTTT9300.1191214218742795No Hit
GCTGCGTTCTTCATCGTTACGGGAACCAAGATATCCATCGTTGAAAATTT8960.11476644516059617No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCATCGTTGAAACTTT8420.10784971743886382No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTCGTTGAAAGTTT8170.10464752867880255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)