FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779711
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA51298265.79129959690194No Hit
TCCGTAGGTGAACCTGCGGAGGGGTCATTACCGAGTTTACAACTCCCAAA214592.7521735617427483No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAAACATCAAA128211.6443271930240821No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG124321.59443691316398No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACAAAACAACAATCTTAA120041.5395447800531223No Hit
TCCGTAGGTGAACCTGCGGAGGGCATCGATGAAGAACGCAGC118621.5213329041144732No Hit
TCCGTAGGTGAACCTGCGGAGCCCCGCATCGATGAAGAACGCAGC80631.034101096431883No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA67050.8599340011876195No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAATGAAAAGTGCTTAAC60850.780417359765349No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACCGAGTTTACAACTCCCAAA53940.6917947803737539No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCATATATCCAATCCAATCTAA36350.46619837349992493No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTCCAACTCCCAAA30790.394889901514792No Hit
TCCGTAGGTGAACCTGCGGCATCGATGAAGAACGCAGC30370.38950329032167047No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTTACAACTCCCAAA28270.36257023435606267No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATAACGTATCCAAGTTTTAA25680.32935279866514644No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCACA24260.31114092272649735No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCCAA21400.2744606655542887No Hit
TCCGTAGGTGAACCTGCGGAGGGAGCATTACCGAGTTTACAACTCCCAAA18310.2348305974906087No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGGTTACAACTCCCAAA17750.22764844923311328No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTACAACTCCCAAA16420.2105908471215617No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG15850.203280446216611No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGATTTTACAACTCCCAAA15650.2007153932675055No Hit
TCCGTAGGTGAACCTGCTGAGGGATCATTACCGAGTTTACAACTCCCAAA15490.1986633509082211No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACACCTCCCAAA15240.19545703472183926No Hit
TCCGTAGGTGAACCTGCGGACTGCATCGATGAAGAACGCAGC14770.18942916029144133No Hit
TCCGTAGGTGAACCTGCGGAGCATCGATGAAGAACGCAGC14020.1798102117322957No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTTCCAAA13650.1750648637764505No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCTCAAA12660.16236785167837828No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGATTACAACTCCCAAA11640.14928608163794022No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAC11010.14120616484825788No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACCACTCCCAAA9920.12722662627563291No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTACCAAA9680.12414856273670628No Hit
TCCGTAGGTGAACCTGCCCAGCATCGATGAAGAACGCAGC9600.12312254155706409No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACCCCCAAA9320.11953146742831638No Hit
TCCGTAGGTGAACCTGCTGTGCACCGTGGTCAACATGCCGCTCACGCTGG8990.11529913006229231No Hit
TCCGTAGGTGCACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA8520.10927125563189438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)