FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001648583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779711
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT45174957.938005235273074No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGGAAGTTT207492.661114182049503No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGTAAGTTT203802.6137889551385065No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG132191.6953717467112814No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA128501.648046519800285No Hit
GCTGCGTTCTTCATCGATGCCCTCCGCAGGTTCACCTACGGA125841.6139313155771817No Hit
GCTGCGTTCTTCATCGATGCCAGAAGCAAGAGATCCGTTGTTGAAAGTTT117231.50350578611819No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT112901.4479723897700558No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGCTT107991.3850003398695159No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGACCCGTTGTTGAAAGTTT102601.3158721628911225No Hit
GCTGCGTTCTTCATCGATGCGGGGCTCCGCAGGTTCACCTACGGA80271.0294840011234934No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT76290.9784394474362937No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG68380.8769916032991711No Hit
GCTGCGTTCTTCATCGTTGTAGGAACCTAGACATCCACCGTTGAAAATTT60030.7699006426740164No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT56970.7306553325527022No Hit
GCTGCGTTCTTCATCGTTGTAGGAACCTAGACATCCACAGTTGAAAATTT41600.5335310134139444No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAATTT37830.4851797653233057No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAACGTTT35200.45144931904256835No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGATAGTTT32130.4120757562737989No Hit
GCTGCGTTCTTCATCGATGCCGCAGGTTCACCTACGGA31310.4015590391824663No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG29670.3805256049998012No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT28320.36321149759333904No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGGTT27750.3559010966883884No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGACAGTTT26730.34281932664795034No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGCAAGTTT25750.33025056719733337No Hit
GCTGCGTTCTTCATCGATGCTAGAACCAAGATATCCGTTGCTAAGAGTTG25650.32896804072278063No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTCGTTGAAAGTTT16840.21597745831468326No Hit
GCTGCGTTCTTCATCGATGCAGTCCGCAGGTTCACCTACGGA16540.21212987889102503No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGCGATCCGTTGTTGAAAGTTT14430.18506857027796195No Hit
GCTGCGTTCTTCATCGATGCTCCGCAGGTTCACCTACGGA14180.18186225409158008No Hit
GCTGCGTTCTTCACCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT13490.17301282141716612No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTTGAAAGTTT10350.13274149011620973No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGCTGTTGAAAGTTT10270.13171546893656752No Hit
GCTGCGTTCTTCATCGATGTAGGAACCTAGACATCCACCGTTGAAAATTT10050.12889391069255146No Hit
GCTGCGTTCTTCATCGATGCTGGGCAGGTTCACCTACGGA9750.1250463312688932No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG9230.11837719360121891No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGCTCCGTTGTTGAAAGTTT9060.11619689859447924No Hit
GCTGCGTTCTTCATCGATGTAGGAACCTAGACATCCACAGTTGAAAATTT8230.1055519288556914No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTCT8200.10516717091332557No Hit
GCTGCGTTCTTCATCGATGTCAGAACCAAGAGATCCGTTGTTGAAAGTTT7980.10234561266930953No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTCCGCAGGTTCACCTACGGA7800.10003706501511458No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)