FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513554
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA18369735.76975352153814No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG14557728.346970328339378No Hit
TCCGTAGGTGAACCTGCGGTTGGATCATTACCAATCTTGACTCGAAAGAG200083.8959875689800882No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG138382.694555976586688No Hit
TCCGTAGGTGAACCTGCGCTGGGGAACAGTGGAGATGAGGGCAGATAGGT73321.4276979636026592No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGATCAT61651.2004579849441344No Hit
TCCGTAGGTGAACCTGCCCAGCATCGATGAAGAACGCAGC36590.712485931372358No Hit
TCCGTAGGTGAACCTGCGGGCATCGATGAAGAACGCAGC32890.6404389801267247No Hit
TCCGTAGGTGAACCTGCGAGGACAATGGCCCATGACATTTCAGTGGCTGC31510.613567414527002No Hit
TCCGTAGGTGAACCTGCGTGCCTGTGGCTCACTCCAGGGCTGTGTTGCGT27830.5419099062610747No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACCGAGTTTACAACTCCCAAA21130.41144650805952243No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTTACTATACTTTTG17020.3314159757299135No Hit
TCCGTAGGTGAACCTGCGGAGCCCCGCATCGATGAAGAACGCAGC16800.3271321029531461No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC16610.3234323946459379No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTATTGATTTGACTATACTTTTG15080.2936400066984193No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTCCAACTCCCAAA12840.2500223929713331No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG11140.21691973969631237No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTTACAACTCCCAAA10560.20562589328483472No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG9250.18011737811408343No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCACA9150.1781701632155528No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCCAA7740.1507144331462709No Hit
TCCGTAGGTGAACCTGCTGAGGGATCATTACCGAGTTTACAACTCCCAAA7460.1452622314303851No Hit
TCCGTAGGTGAACCTGCTGAGGGATCATTAACTGAGAAGAGCGAAGCCGC7330.1427308520622953No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTAACGGAGAAGAGCGAAGCCGC7250.1411730801434708No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTACAACTCCCAAA7030.1368892073667034No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGATTTTACAACTCCCAAA6910.13455254948846665No Hit
TCCGTAGGTGAACCTGCGGAGGGAGCATTACCGAGTTTACAACTCCCAAA6850.13338422054934826No Hit
TCCGTAGGTGAACCTGCGGGTGGATCATTACCAATCTTGACTCGAAAGAG6520.1269584113841972No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGGTTACAACTCCCAAA6500.12656896840449106No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTTCCAAA6250.12170093115816448No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGTCTATACTTTTG6150.11975371625963385No Hit
TCCGTAGGTGAACCTGCGGATGGATCATTACCAATCTTGACTCGAAAGAG5360.10437071856124185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)