FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736920
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA47291664.17467296314389No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACGCCAATTATTCTTCTTGC620648.42208109428432No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAAGTATGGGCCTGCGT326334.428296151549693No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG106761.4487325625576726No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACCGAGTTTACAACTCCCAAA52060.7064538891602887No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG37750.5122672746023992No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTTACAACTCCCAAA31590.428676111382511No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTCCAACTCCCAAA24760.335993052163057No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCACA23960.3251370569396949No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCCAA20180.273842479509309No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGGTTACAACTCCCAAA20150.27343537968843296No Hit
TCCGTAGGTGAACCTGCGGAGGGAGCATTACCGAGTTTACAACTCCCAAA18460.2505020897790805No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTACAACTCCCAAA15920.2160343049449058No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGATTTTACAACTCCCAAA14830.20124301145307494No Hit
TCCGTAGGTGAACCTGCGGGAAGGATCATTAACGGAGCCCGGAGGGCGAG14310.1941866145578896No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGATTACAACTCCCAAA13820.1875373174835803No Hit
TCCGTAGGTGAACCTGCTGAGGGATCATTACCGAGTTTACAACTCCCAAA12350.16758942626065246No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCTCAAA12080.16392552787276773No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACACCTCCCAAA11870.16107582912663518No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTTCCAAA11550.15673343103729034No Hit
TCCGTAGGTGAACCTGCAAAAATTGAGCAATGACCGCATCAGGGTGGGGG11120.1508983336047332No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCAAAAACAAAAACTTGATA10800.14655593551538837No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAC10270.13936383867991098No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTACCAAA9300.12620094447158445No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACCACTCCCAAA9200.12484394506866417No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTCTACAACTCCCAAA7960.1080171524724529No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACCCCCAAA7750.10516745372632036No Hit
TCCGTAGGTGCACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA7700.10448895402486023No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTCACAACTCCCAAA7670.10408185420398416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)