FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633097
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA33182352.41266346231305No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG11141717.598724997907116No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTAGAGTTCCTTCTCGAACAA167822.6507786326581866No Hit
TCCGTAGGTGAACCTGCGGAGCCCCGCATCGATGAAGAACGCAGC135812.1451689077661085No Hit
TCCGTAGGTGAACCTGCGGAGGGCATCGATGAAGAACGCAGC64871.0246455124570168No Hit
TCCGTAGGTGAACCTGCGGCATCGATGAAGAACGCAGC51450.8126716759043242No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACCGAGTTTACAACTCCCAAA34780.5493628938377532No Hit
TCCGTAGGTGAACCTGGCAGGATTCCAACCCCCAGCTCAATAATACTGTA34550.5457299592321556No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG32130.5075051690341291No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTTACAACTCCCAAA22440.35444805456351874No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTCCAACTCCCAAA21820.34465492649625573No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCACA16260.2568326812478972No Hit
TCCGTAGGTGAACCTGCGGCGTGGCATCGATGAAGAACGCAGC15250.24087935971896882No Hit
TCCGTAGGTGAACCTGCGGAGGGAGCATTACCGAGTTTACAACTCCCAAA14480.22871692647414218No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCCAA14180.22397831611901492No Hit
TCCGTAGGTGAACCTGCGGGCGCCTGTGGGGTGGTGTCCGCGCTCGCCCC14060.22208287197696402No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGGTTACAACTCCCAAA12160.19207167306115808No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTTACTATACTTTTG11480.18133082292286964No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTATTGATTTGACTATACTTTTG11020.17406495371167452No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTACAACTCCCAAA10470.1653775013939412No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGATTTTACAACTCCCAAA10390.1641138719659073No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTTCCAAA10010.1581116321827461No Hit
TCCGTAGGTGAACCTGCTGAGGGATCATTACCGAGTTTACAACTCCCAAA9290.14673896733044067No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG8640.13647197822766496No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCTCAAA8550.1350503951211268No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG8100.1279424795884359No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGATTACAACTCCCAAA7750.12241410084078742No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC7500.11846525887818139No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAC7450.11767549048566017No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACACCTCCCAAA7420.11720162945014745No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACCACTCCCAAA7210.11388460220155838No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTACCAAA7000.11056757495296929No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6640.10488124252681658No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)