FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001648590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856240
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC25184429.412781463141176No Hit
TCCGTAGGTGAACCTGCGGAAGGATCAATACATGAATTTGCAAGTGAGAC9524511.12363356068392No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCAGCAGAA8952510.455596561711669No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA544466.358731196860694No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAATTTAGATTGAACCAT403434.7116462674016635No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAATGCCTGGGCTGTCAT306653.581355694665047No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATATAGGACGTCCAAC292023.4104923853125295No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG207522.4236195459217043No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC87851.025997383911053No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGTGCCAGC20940.2445576006727086No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGCTTCAAGGGCCAGC19690.22995889003083247No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA18320.21395870316733628No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17980.20998785387274596No Hit
TCCGTAGGTGAACCTGCCGAAGGATCATTAGTGAAGATTCAAGGGCCAGC16960.19807530598897508No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATGCCTGGGCTGTCAT16680.19480519480519481No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGGAGTCCCGTCGGGAGGGGC15550.1816079603849388No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA13270.15497991217415677No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGCGAAGATTCAAGGGCCAGC12510.1461038961038961No Hit
TCCGTAGGTGAACCTGCGGAAGGATCAATACAGGAATTTGCAAGTGAGAC12200.14248341586471083No Hit
TCCGTAGGTGAACCTGCAGAAGGAGCATTAGTGAAGATTCAAGGGCCAGC11480.1340745585349902No Hit
TCCGTAGGTGAACCTGCAGAAGGATCAATACATGAATTTGCAAGTGAGAC10950.1278847052228347No Hit
TCCGTAGGTGAACCTGCGGCTTCCTGGGAGAGAGGAGAAAAGTCAAAGGC10890.12718396711202468No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTGTCGAAACCTGCCCAGCAGAA10860.12683359805661965No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGA10350.12087732411473419No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCCAGGGCCAGC9850.11503783985798374No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGTCCAGC9840.11492105017284875No Hit
TCCGTAGGTGAACCTGCGGAAGGATCAATACATGAATTTGCAAGTGCGAC9230.1077968793796132No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCCGC9210.10756330000934317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)