FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001648591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856240
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG36787942.96447257778193No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG10654912.443824161450062No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG8587210.028963841913482No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT789739.223231804167057No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG116071.355577875362048No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG112501.31388395776885No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT32030.3740773614874334No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT27550.3217555825469494No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG25950.30306923292534804No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT25040.2924413715780622No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT24130.2818135102307764No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG23480.2742221806970009No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG18060.21092217135382602No Hit
GCTGCGTTCTTCATCGATGGCAGAACCAAGAGATCCGTTGTCGAAAGTTG17830.2082360085957208No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17510.20449873867140053No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTG16310.1904839764551995No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGCGTCG15410.17997290479304867No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGCGAGTCG15110.17646921423899842No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTTGAAAGTTG12730.1486732691768663No Hit
GCTGCGTTCTTCACCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG11190.13068765766607493No Hit
GCTGCGTTCTTCATCGATGCTCACACGTGTACTGAGCAGTGAGAGGGGAA10930.1276511258525647No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT10490.12251237970662432No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG10100.11795758198635896No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGATAGTTG9620.11235167709987853No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGACAGTTG9580.1118845183593385No Hit
GCTGCGTTCTTCATCGATGCGAGAACCGAGATATCCGTTGCCGAGAGTCG8980.10487713725123797No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGCAAGTTG8570.10008876016070262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)