FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689583
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTATCTTA13712419.88506097163068No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG10199214.790387814084744No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA665489.650469921677303No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCACAGCAGAA661639.594639078979615No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTGCCTTTATAGGCTT583168.456704994177642No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC412785.985936428247216No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG252083.6555425525281224No Hit
TCCGTAGGTGAACCTGCAAAAATTGAGCAATGACCGCATCAGGGTGGGGG49180.7131846347720289No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGATCAT47780.6928825101546877No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA45400.6583688983052076No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC38200.5539579717017385No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC28930.41952890369977225No Hit
TCCGTAGGTGAACCTGCGTTGCATCCCAGCGATGAGGCTGACTTGATCAT22490.32613913046000265No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22400.32483399387745926No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA20030.2904653972038174No Hit
TCCGTAGGTGAACCTGCGGAATCTGATGATAAAGCTTTAACATCGTAAAT14170.20548650416266062No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGATAAAACTATTATCTTA14110.2046164131076317No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC13130.19040492587549285No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAACACTATTATCTTA12370.17938377251179335No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC12310.17851368145676447No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTCTCTTA11950.173293135126591No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTTACTATACTTTTG10900.1580665416635851No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTATTGATTTGACTATACTTTTG10270.14893058558578157No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG7580.1099215032853188No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTGTCGAAACCTGCACAGCAGAA7310.10600609353768871No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGATTGCCTTTATAGGCTT6960.10093056238335342No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAACCTATTATCTTA6910.10020548650416267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)