FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689583
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG14596921.16772020191913No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG11589116.80595374305921No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA10728415.557808124620243No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCTAGATATCCGTTGCCGAGAGTCG649669.421055913501348No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT637759.24834283907811No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG136771.9833725599383976No Hit
GCTGCGTTCTTCATCCCGGTTTCAACTCCACTATAGGAACCCTGGAGGAC47790.6930275253305258No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTAAATAAAATACTGCCAAACCG46170.6695350668447453No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG45430.6588039438327221No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC39510.5729549597365364No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG35970.5216195874898308No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA28690.41604853947965653No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA26320.38167994280601464No Hit
GCTGCGTTCTTCATCGATGCGAAAATCCTCAATAAAATACTGGCAAACCG21770.3156980377996557No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21610.3133777949862453No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTG14330.20780674697607107No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG14160.20534148898682247No Hit
GCTGCGTTCTTATCAGACGATGGCAAATTAATGATTGATGATCAACAATA13000.1885197285895969No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA12920.18735960718289169No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT11670.16923271020312275No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAAAGTTA11350.1645922245763019No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG10590.15357107121260238No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGGTG10460.15168587392670643No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT9930.1440000696072844No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG9900.14356502407976993No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAGAGTTA9080.13167377966104155No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT8870.12862846096844033No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTG8090.11731727725306453No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG7660.11108162469202403No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT7460.10818132117526098No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG7150.1036858507242783No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA7060.10238071414173493No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCTAGATATCCGTTGCCGCGAGTCG6910.10020548650416267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)