FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668359
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA14575921.808489150291983No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG11628617.39873331547866No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC10319215.439606558750613No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC415836.221656325417927No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG401936.013684262499645No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACAACATATCCTAACTTAAA315014.713185578409209No Hit
TCCGTAGGTGAACCTGCGGTCCCACAAAATTGGGTGTACTGACCCACTGC64370.9631051575575401No Hit
TCCGTAGGTGAACCTGCGGGCTGACACCAGTTTCGGCGCATCGATGAAGAACGCAGC48740.7292488019163353No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC25180.37674363627930496No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACCGAGTTTACAACTCCCAAA16950.2536062206089841No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTTACAACTCCCAAA16660.24926723512363863No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTTACTATACTTTTG13600.20348345724378664No Hit
TCCGTAGGTGAACCTGCTGAGGGATCATTAACTGAGAAGAGCGAAGCCGC13390.20034143327163995No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTATTGATTTGACTATACTTTTG12850.1922619430575484No Hit
TCCGTAGGTGAACCTGCCACAGCGGAGTAGCGAGGCGGAACAGAGGGGAC12400.18552903454580547No Hit
TCCGTAGGTGAACCTGCATCCCAGGGATTAAGCACACTTGATCTTGGTGG10960.16398372730822808No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10260.15351031406773905No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA9690.14498196328619797No Hit
TCCGTAGGTGAACCTGCAAAAATTGAGCAATGACCGCATCAGGGTGGGGG9260.1385482951527547No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCACA9150.13690247307210646No Hit
TCCGTAGGTGAACCTGCCACAGAGGAGTAGCGAGGCGGAACAGAGGGGAC9100.13615437212635725No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG9050.13540627118060802No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGCTTCAAGGGCCAGC8930.13361082891080991No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTCCAACTCCCAAA8880.13286272796506068No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTAACGGAGAAGAGCGAAGCCGC8200.12268855510287136No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATAAGTAGTTTGCTACT7950.11894805037412529No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG7860.1176014686717767No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGTGCCAGC7030.10518299297233973No Hit
TCCGTAGGTGAACCTGCCGAAGGATCATTAGTGAAGATTCAAGGGCCAGC6740.10084400748699426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)