FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001648595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001648595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668359
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTCT14438321.6026117700218No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG11016316.48260889731417No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA8758813.10493312725646No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC421286.303199328504591No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA368255.5097634654429735No Hit
GCTGCGTTCTTCATCGATGCGAGAATCGAGATATCCATTGTTAACAGTCG300884.50177225114048No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG212823.1842168654869614No Hit
GCTGCGTTCTTCATCCCAATCCAGGCTGCAGCTCCAGATAGGGGACGGTC62700.9381185859695165No Hit
GCTGCGTTCTTCATCGATGCGCCGAAACTGGTGTCAGCCCGCAGGTTCACCTACGGA49050.7338870277799805No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG32530.48671447530443973No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTCT25180.37674363627930496No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT22180.331857579534352No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT16610.2485191341778894No Hit
GCTGCGTTCTTCATCGATGCGAGAACCGAGATATCCATTGTTAACAGTCG14630.2188943367262205No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTCGAAAGTCT13730.20542851970273462No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG13580.20318421686548693No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG11120.16637765033462557No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT10740.16069208314693154No Hit
GCTGCGTTCTTCATCGATTGGAGAACCAAGAGATCCGTTGTCGAAAGTTG10540.15769967936393464No Hit
GCTGCGTTCTTCATCGATGCAAACATTCTCAATAAAATACTGGCAAACCG10480.1568019582290356No Hit
GCTGCGTTCTTCATCGATGCCGGGTGCGAGGAGAACGCGGCCTGGGGCGC10440.15620347747243624No Hit
GCTGCGTTCTTCATCGATGCCCGAGCCGAGTGATCCACCGCTAAGAGTCG10120.15141563141964123No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAAAGTTA10040.1502186699064425No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG9840.14722626612344564No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGCGTCG9790.14647816517769643No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9730.14558044404279735No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT9580.14333614120554972No Hit
GCTGCGTTCTTCATCGATGCGAGAAGCGAGATATCCATTGTTAACAGTCG9430.14109183836830205No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTACGAGTCG9330.13959563647680365No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT9130.13660323269380675No Hit
GCTGCGTTCTTCATCCCGGTTTCAACTCCACTATAGGAACCCTGGAGGAC8410.12583057907501807No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGGCT8330.12463361756181932No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA8210.1228381752920212No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGCTGAAAGTCT7910.11834956961752591No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAACGTCT7760.11610526678027826No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAGAGTTA7700.11520754564537922No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGCGTGATCCACCGCTAAGAGTCG7070.10578147372893908No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGATAGTCT7070.10578147372893908No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTCAGAGTCG6980.1044348920265905No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG6890.10308831032424191No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGTCG6730.10069438729784443No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTCG6700.10024552673039488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)