FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001671010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001671010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26224547
Sequences flagged as poor quality0
Sequence length101
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT555880.21196934307387655TruSeq Adapter, Index 13 (97% over 40bp)
CGATAACGTTGTAGATGTGGTCGTTACCTAGAAGGTTGCCTGGCTGGCCC382240.14575656921738248No Hit
AAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGTGTATGCATCG309860.11815647378008093No Hit

[OK]Adapter Content

Adapter graph