FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001671032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001671032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15816188
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1273060.8049095015815442TruSeq Adapter, Index 5 (100% over 50bp)
GCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGACATAACTAT370150.23403237240225014No Hit
CTTCGATAATGGCCCATTTGGGCAAAAAGCCGGTTAGCGGGGGCAGGCCT259970.1643695686975901No Hit
AAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGTGTATGCATCG187540.11857471598086719No Hit
GTCGGTTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAAT169920.10743423130782209No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)