FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001671046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001671046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6822223
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT683651.0020927196311231TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA651540.9550259497527419TruSeq Adapter, Index 15 (97% over 40bp)
CAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGT136300.19978825083847304No Hit
CAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCG123850.18153906725124638No Hit
GCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGACATAACTAT112340.16466773366980234No Hit
TATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAG110050.16131105652805544No Hit
TTTCGATAATAACTAGTATGGGGATAAGGGGTGTAGGTGTGCCTTGTGGT73100.10714982491777239No Hit
TGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAG70090.10273777330351119No Hit
CTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCC69910.1024739296853826No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)