FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001671047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001671047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7722478
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC654800.8479143611674905Illumina Single End PCR Primer 1 (100% over 50bp)
GCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGACATAACTAT193240.25023056070862226No Hit
CAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGT119200.1543545996505267No Hit
CAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCG104500.1353192589218124No Hit
TATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAG93540.12112692324924719No Hit
TGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAG90420.11708676929866293No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)