FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001671072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001671072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9632891
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT683210.7092470993391289TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA196850.20435194377264312TruSeq Adapter, Index 13 (97% over 40bp)
CAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGT97350.10106000368944276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)