FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001671440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001671440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8623391
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATT269080.3120350219536607No Hit
GGGAAGGTTAATGGTTGATATTGCTAGGGCGGTGCTTCCAATTAGGTGCA138390.16048211196732234No Hit
CTGACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAG105330.12214452528013632No Hit
GTAGAATTAGAATTGTGAAGATGATAAGTGTAGAGGGAAGGTTAATGGTT99770.11569694566789328No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC91840.10650102726410063TruSeq Adapter, Index 7 (100% over 50bp)
CCTGGCCGTACGCCTGACCGCTAACATTACTGCAGGCCACCTACTCATGC91170.10572407072809292No Hit
CGCCTGACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGG87640.10163055345629114No Hit
GGTTGATATTGCTAGGGCGGTGCTTCCAATTAGGTGCATGAGTAGGTGGC87080.10098115694858321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)