FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001671448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001671448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8222118
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA339490.4128984770104248TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT305350.3713763290675225TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph