FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001671514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001671514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5983292
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT418690.6997652797155813TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA386230.6455142085661204TruSeq Adapter, Index 15 (97% over 40bp)
GCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGACATAACTAT65470.10942136870471975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)