FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001676522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001676522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16230526
Sequences flagged as poor quality0
Sequence length126
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTATAGATCTCGTAT10310726.352671503067738TruSeq Adapter, Index 13 (97% over 38bp)
CGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGG704190.4338676392866134No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTATAGATATCGTAT463120.2853388731825451TruSeq Adapter, Index 13 (97% over 38bp)
CGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATC274330.16902101632442473No Hit
CTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGA251070.15468999587567278No Hit
CGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGC208990.1287635409967613No Hit
CCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGG200730.12367436520541603No Hit
GCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGC191170.11778422954376218No Hit
CCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTG190790.11755010281244119No Hit
GGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGG190660.11747000682541034No Hit
CGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCG189100.11650885498104005No Hit
GATCGGAAGAGCCCACGTCTGAACTCCAGTCACAGTTATAGATCTCGTAT187200.11533822132443519TruSeq Adapter, Index 14 (97% over 37bp)
CGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGG182230.11227609012794779No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAGTTATAGATCTCGTATG169480.10442052216915214TruSeq Adapter, Index 14 (96% over 32bp)
CGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGC167530.10321908236368925No Hit
GCCGCCCCCCCCACGCCCGGGGCACCCCCCTCGCGGCCCTCCCCCGCCCC165260.10182048320553506No Hit
GCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGT164860.10157403401467088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)