FastQCFastQC Report
Wed 14 Sep 2022
EGAF00001676524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001676524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17231610
Sequences flagged as poor quality0
Sequence length126
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCGATTCATCTCGTAT9114215.289238788482329TruSeq Adapter, Index 1 (97% over 36bp)
CTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGA536190.3111665131696922No Hit
GCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGC429350.24916418140846966No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCGATTCATATCGTAT375350.2178264248088252TruSeq Adapter, Index 1 (97% over 36bp)
CGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGG368950.21411232032294136No Hit
CGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCG320920.18623912681403537No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT186280.1081036536922551No Hit
CGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCG182240.10575912523554097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)