FastQCFastQC Report
Tue 27 Sep 2022
EGAF00001676526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001676526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24870880
Sequences flagged as poor quality0
Sequence length126
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAGAATATCTCGTAT20692038.319782010125898TruSeq Adapter, Index 1 (97% over 37bp)
CGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGG1663340.6687901674568812No Hit
CCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGG634710.2552020676389416No Hit
CGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGG558920.22472867867964463No Hit
CGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGC537450.21609609310165143No Hit
CGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATC530900.21346249107389845No Hit
CCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTG498590.20047139465913552No Hit
GGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGG461510.18556239264553567No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC458510.18435616270916028No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG442980.17811191240519034No Hit
CGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGC417140.16772225188654363No Hit
CGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCG403990.16243494399876482No Hit
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC403160.16210122038303432No Hit
CGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCA390370.15695866008762052No Hit
GCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAA388480.15619873522770403No Hit
GATCGGAAGAGCCCACGTCTGAACTCCAGTCACATTAGAATATCTCGTAT379960.15277304220839794TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACATTAGAATATCTCGTATG360850.14508935751368668TruSeq Adapter, Index 27 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAGAATATATCGTAT356000.14313928578321314TruSeq Adapter, Index 1 (97% over 37bp)
GCCGCCCCCCCCACGCCCGGGGCACCCCCCTCGCGGCCCTCCCCCGCCCC344160.13837869830098493No Hit
CGCCGCCCCCCCCACGCCCGGGGCACCCCCCTCGCGGCCCTCCCCCGCCC343590.1381495146130736No Hit
GCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGT339880.1366578102584227No Hit
GCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCG296740.11931222377334458No Hit
CCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCA292990.11780443635287534No Hit
CTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTG292310.11753102423396357No Hit
GCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCG291930.11737823510868936No Hit
GGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGC287730.1156895131977638No Hit
CGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCG252440.10150022837953462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)