FastQCFastQC Report
Sat 1 Apr 2023
EGAF00001676544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001676544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18328436
Sequences flagged as poor quality0
Sequence length126
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTTCATATCTCGTAT9282505.064534693522131TruSeq Adapter, Index 14 (97% over 37bp)
CTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGA467140.2548717195509753No Hit
GCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCC449080.24501817831046796No Hit
CGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGG438800.23940940732749919No Hit
CGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCG411780.2246672874870502No Hit
GCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGC400520.2185238282197128No Hit
CGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCG318450.17374641240529198No Hit
CAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCT249790.13628549648207844No Hit
GTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGAC217680.11876627116465366No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTTCATATATCGTAT206970.11292289205691092TruSeq Adapter, Index 14 (97% over 37bp)
GCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCG195080.10643570460676513No Hit
GCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGC188620.10291112673225364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)