FastQCFastQC Report
Fri 31 Mar 2023
EGAF00001676546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001676546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13410909
Sequences flagged as poor quality0
Sequence length126
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATATAAGAATCTCGTAT7677505.724817012776688TruSeq Adapter, Index 15 (97% over 37bp)
CGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGG557120.41542299630845303No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATATAAGAATATCGTAT282990.21101477908768151TruSeq Adapter, Index 15 (97% over 37bp)
CTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGA280040.2088150773374124No Hit
CGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATC274170.20443804368518195No Hit
CGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCG263350.19636998506216097No Hit
GCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGC235220.17539452396552688No Hit
GCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCC206750.15416553792140414No Hit
CGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGC178790.13331683929851434No Hit
CCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCA174610.13019997376762454No Hit
GGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGG165880.12369034791004845No Hit
CGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCG156740.11687500079226545No Hit
CCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGG145690.10863543999888449No Hit
GCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGT145640.10859815691837145No Hit
GCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCC143590.10706955061733697No Hit
GATCGGAAGAGCCCACGTCTGAACTCCAGTCACATATAAGAATCTCGTAT142640.1063611720875893TruSeq Adapter, Index 1 (97% over 35bp)
CGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCG138760.1034680050397777No Hit
CCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTG134600.10036605274109309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)