FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001676548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001676548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12182548
Sequences flagged as poor quality0
Sequence length126
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTTATTATCTCGTAT7430726.09947935358022TruSeq Adapter, Index 15 (97% over 37bp)
CGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGG1871171.5359430555906697No Hit
CCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTG658480.540510901331971No Hit
GGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGG506140.4154631691170025No Hit
CGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATC474400.3894095061230212No Hit
CGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGC449290.36879805439715896No Hit
GCCGCCCCCCCCACGCCCGGGGCACCCCCCTCGCGGCCCTCCCCCGCCCC306450.25154836246079226No Hit
GCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCG300710.24683670443982653No Hit
CGCCGCCCCCCCCACGCCCGGGGCACCCCCCTCGCGGCCCTCCCCCGCCC300170.24639344741346392No Hit
GCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCT268970.22078304144584532No Hit
CGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCG257720.21154852006329056No Hit
GCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCC246860.202634128755331No Hit
GCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGT240610.19750383909835612No Hit
GCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGG236930.1944831245483293No Hit
CCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCA225830.18537173011754193No Hit
CGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCG224440.1842307536978307No Hit
GCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCC218780.17958476338447424No Hit
CCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGAT212800.17467610224068067No Hit
GTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGAC194980.1600486203707139No Hit
CGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGC194340.15952327870983968No Hit
GCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCC193640.15894868626825848No Hit
CCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGG188710.1549019137868367No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTTATTATATCGTAT183890.15094543440337768TruSeq Adapter, Index 15 (97% over 37bp)
CGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCG179250.14713670736203951No Hit
GCCCCCCCCACGCCCGGGGCACCCCCCTCGCGGCCCTCCCCCGCCCCACC161280.13238609854030536No Hit
CTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTG159730.13111378670537557No Hit
GCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCG158230.12988251718770163No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC156550.12850349532790678No Hit
CGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGG142900.11729894271707364No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG138090.11335067179706577No Hit
CAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCT137460.1128335385996427No Hit
GCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAA137240.11265295240371717No Hit
GCCGCCGCCCCCCCCACGCCCGGGGCACCCCCCTCGCGGCCCTCCCCCGC137160.11258728469610792No Hit
GATCGGAAGAGCCCACGTCTGAACTCCAGTCACATCTTATTATCTCGTAT136270.11185673144895468TruSeq Adapter, Index 1 (97% over 36bp)
CTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCC131300.10777712511372825No Hit
CGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGG130370.10701373801277039No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACATCTTATTATCTCGTATG126930.10419002658557143TruSeq Adapter, Index 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)