FastQCFastQC Report
Sun 14 May 2023
EGAF00001676550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001676550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27884882
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC17181886.161718740642331TruSeq Adapter, Index 8 (100% over 50bp)
GCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGC762930.27359986676651527No Hit
CGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGG733370.2629991405378728No Hit
GCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGG544320.19520254738750553No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT475280.17044361170328784No Hit
GCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCG442920.15883875714446272No Hit
CGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGG440770.15806773003378677No Hit
CTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGA425940.15274943605642657No Hit
CCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCC407060.14597874217290932No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGC349340.1252793538807157TruSeq Adapter, Index 8 (98% over 50bp)
CCGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGAC339370.12170393979074397No Hit
GGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCT338510.12139552894647357No Hit
CGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGG318930.11437380298041067No Hit
GACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGG313720.11250540705174941No Hit
GGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGG313430.11240140804612334No Hit
GGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGAT299910.1075529026803843No Hit
CCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTG297630.10673525532580702No Hit
CTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTG283440.10164647639534569No Hit
GCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCC282860.10143847838409356No Hit
CCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCC281740.10103682705202052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)