FastQCFastQC Report
Thu 9 May 2024
EGAF00001682375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4863340
Total Bases364.7 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCACAAACATCTCGTATGCC460710.9473119296615083Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCACAAACATCTCGTATGC114470.23537322087289805Illumina RNA RT Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)