FastQCFastQC Report
Wed 8 May 2024
EGAF00001682377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938044
Total Bases220.3 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCC2008486.8361127335056935Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGC580211.9748172593739237Illumina RNA RT Primer (95% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCG131480.4475086145748668Illumina RNA RT Primer (95% over 22bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACA127180.4328730270887707Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTG79920.2720177097415832TruSeq Adapter, Index 2 (95% over 24bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATC62720.2134753597971984Illumina RNA RT Primer (95% over 22bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTC60950.2074509435529216Illumina RNA RT Primer (95% over 22bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATG58910.20050754855951786Illumina RNA RT Primer (95% over 22bp)
ACCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA49760.16936437983910385Illumina RNA RT Primer (95% over 22bp)
GCCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA48690.16572250109256362Illumina RNA RT Primer (95% over 22bp)
CCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTAT44200.1504402248570818Illumina RNA RT Primer (95% over 22bp)
ACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTAT40340.13730223236956288Illumina RNA RT Primer (95% over 22bp)
GACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA40230.13692783361991856Illumina RNA RT Primer (95% over 22bp)
CACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA37960.12920160487725846Illumina RNA RT Primer (95% over 22bp)
ATCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA36340.12368773238249665Illumina RNA RT Primer (95% over 22bp)
ATGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATG35580.12110097738495407Illumina RNA RT Primer (95% over 22bp)
AACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA31720.10796298489743517Illumina RNA RT Primer (95% over 22bp)
GAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCG29720.10115573490390205No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)