FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001682377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938044
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1860686.333056958983596TruSeq Adapter, Index 2 (96% over 28bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG531041.807461018282912RNA PCR Primer, Index 21 (96% over 27bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA128390.43699141333485814RNA PCR Primer, Index 21 (96% over 27bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCT116350.3960117683737888RNA PCR Primer, Index 21 (96% over 25bp)
CGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG74980.2552038022575564TruSeq Adapter, Index 2 (96% over 28bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAA61010.2076551610527276RNA PCR Primer, Index 21 (96% over 27bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA59590.2028220135573191RNA PCR Primer, Index 21 (96% over 27bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG52640.17916681982979152RNA PCR Primer, Index 21 (96% over 27bp)
ACCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAA46000.1565667498512616RNA PCR Primer, Index 21 (96% over 27bp)
GCCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAA45080.15343541485423637RNA PCR Primer, Index 21 (96% over 27bp)
CCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAA41860.14247574236464805RNA PCR Primer, Index 21 (96% over 27bp)
ACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAA38200.13001847487648244RNA PCR Primer, Index 21 (96% over 27bp)
GACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAA37880.12892931487751716RNA PCR Primer, Index 21 (96% over 27bp)
CACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAA35490.12079465113524507RNA PCR Primer, Index 21 (96% over 27bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGT34550.11759524363828451TruSeq Adapter, Index 2 (96% over 28bp)
ATCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAA33950.11555306864022459RNA PCR Primer, Index 21 (96% over 27bp)
ATGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG32070.10915425364630346RNA PCR Primer, Index 21 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)