FastQCFastQC Report
Wed 8 May 2024
EGAF00001682378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2864312
Total Bases214.8 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCC1977006.902181047316075Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGC564631.9712587176257335Illumina RNA RT Primer (95% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCG130630.4560606526104698Illumina RNA RT Primer (95% over 22bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACA124300.43396110479584626Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTG76760.2679875656003955TruSeq Adapter, Index 2 (95% over 24bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATC59620.208147715751636Illumina RNA RT Primer (95% over 22bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTC58750.20511033714204321Illumina RNA RT Primer (95% over 22bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATG55120.19243713673650079Illumina RNA RT Primer (95% over 22bp)
ACCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA47550.1660084515932622Illumina RNA RT Primer (95% over 22bp)
GCCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA46130.16105089110404175Illumina RNA RT Primer (95% over 22bp)
CCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTAT41990.14659715841011733Illumina RNA RT Primer (95% over 22bp)
ACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTAT39940.13944011685877797Illumina RNA RT Primer (95% over 22bp)
GACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA39260.13706607380760197Illumina RNA RT Primer (95% over 22bp)
CACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA35260.12310111468303733Illumina RNA RT Primer (95% over 22bp)
ATGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATG34350.11992408648219886Illumina RNA RT Primer (95% over 22bp)
ATCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA34090.11901636413910216Illumina RNA RT Primer (95% over 22bp)
AACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA30450.10630825133574834Illumina RNA RT Primer (95% over 22bp)
GAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCG29550.10316613553272129No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)