FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001682378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2864312
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1829776.388165814338661TruSeq Adapter, Index 2 (96% over 28bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG515351.7992104212110969RNA PCR Primer, Index 21 (96% over 27bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA127690.4457964076539148RNA PCR Primer, Index 21 (96% over 27bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCT114060.39821080943696074RNA PCR Primer, Index 21 (96% over 25bp)
CGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG71410.24930943277129028TruSeq Adapter, Index 2 (96% over 28bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAA57910.20217769572588462RNA PCR Primer, Index 21 (96% over 27bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA57290.20001312706157706RNA PCR Primer, Index 21 (96% over 27bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG49290.1720832088124478RNA PCR Primer, Index 21 (96% over 27bp)
ACCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAA44110.1539985867461366RNA PCR Primer, Index 21 (96% over 27bp)
GCCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAA42600.14872681467661344RNA PCR Primer, Index 21 (96% over 27bp)
CCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAA39770.13884660609598395RNA PCR Primer, Index 21 (96% over 27bp)
ACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAA37970.13256237448992986RNA PCR Primer, Index 21 (96% over 27bp)
GACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAA36870.1287220107306746RNA PCR Primer, Index 21 (96% over 27bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGT34140.11919092612815922TruSeq Adapter, Index 2 (96% over 28bp)
CACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAA32770.11440792762799584RNA PCR Primer, Index 21 (96% over 27bp)
ATCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAA32250.11259248294180242RNA PCR Primer, Index 21 (96% over 27bp)
ATGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG30580.10676211250729668RNA PCR Primer, Index 21 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)